COMPARAÇÃO DA ADESÃO MICROBIANA EM DIFERENTES MATERIAIS USADOS NO SELAMENTO DE PERFURAÇÕES RADICULARES
DOI:
https://doi.org/10.24980/ucm.v11i13.5622Palavras-chave:
Endodontia, Trióxido mineral, Bio-C Repair, Enterococcus faecalisResumo
O agregado de trióxido mineral (MTA) é muito utilizado na endodontia por estimular a formação de tecido mineralizado e por apresentar atividade antimicrobiana contra bactérias orais. Porém, apresenta desvantagens como descoloração do elemento dental e dificuldade de manipulação, fatores que estimulam o desenvolvimento de novos materiais como o Bio-C Repair. O Bio-C Repair e MTA são materiais reparadores utilizados em situações de selamentos de perfurações, onde a formação de um biofilme pode levar à perda do elemento dental. Assim, o objetivo desse estudo foi avaliar a adesão microbiana do Enterococcus faecalis sobre os materiais reparadores. Para isso, oito discos (1x1mm) de cada material (MTA, Bio-C Repair, Cimento de Ionômero de Vidro e Resina) foram preparados, de acordo com o fabricante, e colocados em placas contendo inóculo de E. faecalis (ATCC 29212) à 37ºC em estufa no período de 7 dias, para induzir a formação de biofilme. Após o tempo experimental (7 dias), os discos foram lavados em solução salina, agitados em vortex, diluídos e inoculadas em placa petri contendo meio de M-Enterococcus em triplicada para a avaliação da atividade antimicrobiana. As unidades formadoras de colônia foram contadas e submetidas a análise estatisticamente. Os resultados mostraram que não houve diferença estatística na adesão microbiana entre o MTA e o Bio-C Repair, aceitando a hipótese nula, porém, ambos os materiais apresentaram uma superfície regular, mas não uniforme o que poderia explicar a adesão microbiana. Diante desses resultados, o Bio-C Repair apresenta o mesmo comportamento microbiano que o MTA.
COMPARISON OF MICROBIAL ADHESION ON DIFFERENT SEALANTS FOR ROOT CANAL PERFORATIONS
ABSTRACT
Mineral trioxide aggregate (MTA) is widely used in endodontics as it stimulates the formation of mineralized tissue and for presenting antimicrobial activity against oral bacteria. However, it has some disadvantages such as dental element discoloration and it is difficult to manipulate, factors that stimulate the development of new fillings such as Bio-C Repair. Bio-C Repair and MTA are repair aids used in perforation sealing situations, where the formation of a biofilm may result in the loss of the dental element. Hence, the aim of this study was to evaluate the microbial adhesion of Enterococcus faecalis on repair materials. For this purpose, eight discs (1x1mm) of each filling material (MTA, Bio-C Repair, Glass Ionomer Cement and Resin) were prepared as prescribed by the manufacturer and placed on plates containing inoculum of E. faecalis (ATCC 29212) at 37ºC in an incubator for 7 days to induce biofilm formation. Subsequent to the experimental time (7 days), the discs were washed in saline, vortex-stirred, diluted, and inoculated into petri dishes containing M-Enterococcus culture medium in triplicate for the evaluation of antimicrobial activity. The colony forming units were counted and statistically analyzed. The results revealed that there was no statistical difference in microbial adhesion between MTA and Bio-C Repair, accepting the null hypothesis, however, both filling materials presented a regular but not uniform surface which may explain microbial adhesion. In view of these results, Bio-C Repair presented the same microbial behavior as MTA.
Keywords: Endodontics. Mineral trioxide. Bio-C Repair. Enterococcus faecalis.
Referências
BACHOO, I. K.; SEYMOUR, D.; BRUNTON, P. A biocompatible and bioactive replacement for dentine: is this a reality? The properties and uses of a novel calcium-based cement. British Dental Journal, v. 214, n. 2, p. 1-7, 2013. Disponível em: https://doi.org/10.1038/sj.bdj.2013.57.
BENETTI, F. et al. Cytotoxicity, Biocompatibility and Biomineralization of a New Ready-for-Use Bioceramic Repair Material. Brazilian Dental Journal, v. 30, n. 4, p. 325-332, 2019. Disponível em: https://doi.org/10.1590/0103-6440201902457.
BHAVANA, V. et al. Evaluation of antibacterial and antifungal activity of new calcium-based cement (Biodentine) compared to MTA and glass ionomer cement. Journal of conservative dentistry, v. 18, n. 1, p. 44–46, 2015. Disponível em: https://doi.org/10.4103/0972-0707.148892.
ESTRELA, C. et al. Antimicrobial and chemical study of MTA, Portland Cement, Calcium Hydroxide Paste, Sealapex and Dycal. Brazilian Dental Journal, v. 11, n. 1, p. 3-9, 2000. Disponível em: http://143.107.206.201/bdj/bdj11(1)/t01111/t01111.html.
ESTRELA, C. et al. Root perforations: a review of diagnosis, prognosis and materials. Brazilian Oral Research, v. 32, suppl. 1, e73, p. 133-146, 2018. Disponível em: https://doi.org/10.1590/1807-3107bor-2018.vol32.0073.
FARIA-JÚNIOR, N. B. et al. Antibiofilm activity, pH and solubility of endodontic sealers. International Endodontic Journal, v. 46, n. 8, p. 755-762, 2013. Disponível em: https://doi.org/10.1111/iej.12055.
GHILOTTI, J. et al. Comparative Surface Morphology, Chemical Composition, and Cytocompatibility of Bio-C Repair, Biodentine, and ProRoot MTA on hDPCs. Materials (Basel), v. 13, n. 9, 2189, p. 1-13, 2020. Disponível em: https://doi.org/10.3390/ma13092189.
HA, W. N. et al. Mineral Trioxide Aggregate-A Review of Properties and Testing Methodologies. Materials (Basel), v. 10, n. 11, 1261, p. 1-18, 2017. Disponível em: https://doi.org/10.3390/ma10111261.
HAAPASALO, H. K. et al. Inactivation of local root canal medicaments by dentine: an in vitro study. International Endodontic Journal, v. 33, n. 2, p. 126–131, 2000. Disponível em: https://doi.org/10.1046/j.1365-2591.2000.00291.x.
HAN, L.; OKIJI, T. Uptake of calcium and silicon released from calcium silicate-based endodontic materials into root canal dentine. International Endodontic Journal, v. 44, n. 12, p. 1081-1087, 2011. Disponível em: https://doi.org/10.1111/j.1365-2591.2011.01924.x.
JACOBOVITZ, M.; LIMA, R. K. de. Treatment of inflammatory internal root resorption with mineral trioxide aggregate: a case report. International Endodontic Journal, v. 41, n. 10, p. 905–912, 2008. Disponível em: https://doi.org/10.1111/j.1365-2591.2008.01412.x.
KIMYAI, S. et al. Effect of two prophylaxis methods on adherence of Streptococcus mutans to microfilled composite resin and giomer surfaces. Medicina Oral Patologia Oral y Cirugia Bucal. v. 16, n. 4, e561-e567, 2011. Disponível em: https://doi.org/10.4317/medoral.16.e561.
KORUYUCU, M. et al. An assessment of antibacterial activity of three pulp capping materials on Enterococcus faecalis by a direct contact test: An in vitro study. European Journal of Dentistry, v. 9, n. 2, p. 240-245, 2015. Disponível em: https://doi.org/10.4103%2F1305-7456.156837.
LODIENE, G. et al. Sealing ability of mineral trioxide aggregate, glass ionomer cement and composite resin when repairing large furcal perforations. British Dental Journal, v. 210, n. 5, p. 1-6, E7, 2011. Disponível em: https://doi.org/10.1038/sj.bdj.2011.198.
MELO, P. A. V. et al. Perfuração radicular cervical: relato de um caso clínico. Revista de Odontologia da Universidade Cidade de São Paulo, v. 23, n. 3, p. 266-272, 2017. Disponível em: https://doi.org/10.26843/ro_unicid.v23i3.386.
MORETTON T. R. et al. Tissue reactions after subcutaneous and intraosseous implantation of mineral trioxide aggregate and ethoxybenzoic acid cement. Journal of Biomedical Materials Research. v. 52, n. 3, p.528-533, 2000. Disponível em: https://doi.org/10.1002/1097-4636(20001205)52:3%3C528::AID-JBM11%3E3.0.CO;2-9.
NOWICKA, A. et al. Response of human dental pulp capped with biodentine and mineral trioxide aggregate. Journal of Endodontics, v. 39, n. 6, p.743–747, 2013. Disponível em: https://doi.org/10.1016/j.joen.2013.01.005.
PARIROKH, M.; TORABINEJAD, M. Mineral trioxide aggregate: a comprehensive literature review-Part I: chemical, physical, and antibacterial properties. Journal of Endodontics, v. 36, n. 1, p. 16-27, 2010. Disponível em: https://doi.org/10.1016/j.joen.2009.09.006.
PORTENEIER, I.; WALTIMO, T. M.T; HAAPASALO, M. Enterococcus faecalis – the root canal survivor and ‘star’ in post-treatment disease. Endodontic Topics. v. 6, n. 1, p. 135-159, 2003. Disponível em: https://doi.org/10.1111/j.1601-1546.2003.00040.x.
ROBERTS, H. W. et al. Mineral trioxide aggregate material use in endodontic treatment: a review of the literature. Dental Materials, v. 24, n. 2, p. 149-164, 2008. Disponível em: https://doi.org/10.1016/j.dental.2007.04.007.
TOUBES, K. S.de. et al. Bio-C Repair - A New Bioceramic Material for Root Perforation Management: Two Case Reports. Brazilian Dental Journal, v. 32, n. 1, p. 104-110, 2021. Disponível em: https://doi.org/10.1590/0103-6440202103568.
VANNI, J. R. et al. Radiographic evaluation of furcal perforations sealed with different materials in dogs' teeth. Journal of Applied Oral Science. v. 19, n. 4, p. 421-425, 2011. Disponível em: https://doi.org/10.1590/S1678-77572011005000019.
WERLANG, A. I. et al. Insucesso no tratamento endodôntico: uma revisão de literatura. Revista Tecnológica, v. 5, n. 2, p. 31-47, 2016. Disponível em: https://uceff.edu.br/revista/index.php/revista/article/view/146.
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Copyright (c) 2022 Julia de Oliveira NEVES, Franciele de Oliveira DIAS, Laís Stabile PRONI, Maria Letícia Oliveira e FREITAS, Carolina Simonetti LODI, Gabriely Cristinni REZENDE (Autor)
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